Cationic Colloidal Gold
Item# BC-CAT
Brand: Biocell

BioSite Cationic Colloidal Gold allows highly sensitive and discrete microscopical studies of anionic sites in and on cells and tissues. A simple one step incubation of sections with the gold conjugate reveals subcellular sites having net negative charge. The charge distribution may be examined with clear definition at a range of magnifications in the electron microscope by employing cationic gold of different sizes.

Most cells of eukariotic origin have a net negative surface charge from anionic plasma membrane components. This charge distribution is thought to be important in the movement of various soluble macromolecules across cell walls. The role of the surface charge in cellular behavior through interaction with neighboring cells or with the intracellular matrix can now be studied microscopically with high definition by a simple one step procedure. A further great advantage of cationic colloidal gold is the possibility of performing labeling studies under various physiological conditions of pH and ionic strength.

FeaturesSpecifications

For high resolution electron microscope studies, BioSite gold conjugates offer the best quality in size distribution, freedom from clustering and sensitivity. In addition, because of the exceptionally high concentration of the conjugates as supplied, very small amounts of material (e.g. 1?) are required for each incubation, making the technique a very inexpensive and efficient one. Immunogold conjugates are made from the highest quality components. All BioSite conjugated immunoglobulins are affinity purified and monospecific, giving very low cross reactivity.